3PKD
M. tuberculosis MetAP with bengamide analog Y10, in Mn form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Collection date | 2009-10-31 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 57.527, 79.849, 64.644 |
| Unit cell angles | 90.00, 91.64, 90.00 |
Refinement procedure
| Resolution | 38.250 - 1.470 |
| R-factor | 0.1605 |
| Rwork | 0.159 |
| R-free | 0.19200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.028 |
| RMSD bond angle | 2.393 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 1.470 |
| Number of reflections | 49388 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 298 | 0.1M Bis-Tris, pH 5.5, 1.3M AMS, 10% glycerol, vapor diffusion, hanging drop, temperature 298K, VAPOR DIFFUSION, HANGING DROP |
