3PK8
Urate oxidase under 0.5 MPa / 5 bars pressure of nitrous oxide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM30A |
Synchrotron site | ESRF |
Beamline | BM30A |
Temperature [K] | 298 |
Detector technology | CCD |
Collection date | 2010-09-17 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9797 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 80.290, 96.264, 105.562 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.480 - 1.650 |
R-factor | 0.16714 |
Rwork | 0.166 |
R-free | 0.18854 |
Structure solution method | RIGID BODY |
Starting model (for MR) | 2iba |
RMSD bond length | 0.012 |
RMSD bond angle | 1.469 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.740 |
High resolution limit [Å] | 1.650 | 1.650 |
Number of reflections | 49385 | |
<I/σ(I)> | 20.3 | 6.2 |
Completeness [%] | 99.9 | 99.6 |
Redundancy | 4.7 | 4.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | BATCH | 8.5 | 298 | 10-15 mg.ml-1 Urate oxidase, 2 mg.ml-1 8-azaxanthine, 50 mM Tris/HCl pH 8.5, 5-8 % PEG 8000, 0-0.05 M NaCl, BATCH, temperature 298K |