3PK5
Urate oxidase under 0.1 MPa / 1 bar pressure of xenon
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM30A |
| Synchrotron site | ESRF |
| Beamline | BM30A |
| Temperature [K] | 298 |
| Detector technology | CCD |
| Collection date | 2010-09-17 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9797 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 80.308, 96.232, 105.558 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.750 - 1.750 |
| R-factor | 0.16259 |
| Rwork | 0.161 |
| R-free | 0.18405 |
| Structure solution method | RIGID BODY |
| Starting model (for MR) | 2iba |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.504 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.840 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Number of reflections | 41205 | |
| <I/σ(I)> | 18.9 | 4.3 |
| Completeness [%] | 99.3 | 95.9 |
| Redundancy | 4.7 | 4.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | BATCH | 8.5 | 298 | 10-15 mg.ml-1 Urate oxidase, 2 mg.ml-1 8-azaxanthine, 50 mM Tris/HCl pH 8.5, 5-8 % PEG 8000, 0-0.05 M NaCl, BATCH, temperature 298K |
