3PK4
Urate oxidase under 3.2 MPa / 32 bars pressure of xenon
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM30A |
| Synchrotron site | ESRF |
| Beamline | BM30A |
| Temperature [K] | 298 |
| Detector technology | CCD |
| Collection date | 2010-09-17 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9797 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 80.405, 96.311, 105.593 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.760 - 1.850 |
| R-factor | 0.16109 |
| Rwork | 0.159 |
| R-free | 0.19341 |
| Structure solution method | RIGID BODY |
| Starting model (for MR) | 2iba |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.546 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.950 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Number of reflections | 35288 | |
| <I/σ(I)> | 15.3 | 5.1 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 4.8 | 4.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | BATCH | 8.5 | 298 | 10-15 mg.ml-1 Urate oxidase; 2 mg.ml-1 8-azaxanthine; 50 mM Tris/HCl pH 8.5; 5-8 % PEG 8000; 0-0.05 M NaCl. , BATCH, temperature 298K |
