3PJY

Crystal structure of a putative transcription regulator (R01717) from Sinorhizobium meliloti 1021 at 1.55 A resolution

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	CCD
Collection date	2010-02-05
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	0.91162,0.97980,0.97961
Spacegroup name	P 43 21 2
Unit cell lengths	63.435, 63.435, 153.938
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.208 - 1.550
R-factor	0.1626
Rwork	0.161
R-free	0.19030
Structure solution method	MAD
RMSD bond length	0.018
RMSD bond angle	1.600
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	SHELX
Refinement software	REFMAC (5.5.0110)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	29.208	29.208	1.610
High resolution limit [Å]	1.550	3.330	1.550
Rmerge	0.039	0.020	0.576
Number of reflections	45713	7748	9108
<I/σ(I)>	13.56	42.7	1.7
Completeness [%]	96.0	87.9	97.2
Redundancy	4.797

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.2	277	0.20M (NH4)2SO4, 10.00% Glycerol, 20.00% PEG-300, 0.1M Phosphate Citrate pH 4.2, NANODROP, VAPOR DIFFUSION, SITTING DROP, temperature 277K