3PJV
Structure of Pseudomonas fluorescence LapD periplasmic domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE A1 |
| Synchrotron site | CHESS |
| Beamline | A1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-02-01 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.9769 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 41.488, 71.408, 110.412 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.080 - 1.773 |
| R-factor | 0.204 |
| Rwork | 0.203 |
| R-free | 0.22400 |
| Structure solution method | SAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.974 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((phenix.refine: 1.6_289)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.080 | 1.830 |
| High resolution limit [Å] | 1.773 | 1.773 |
| Number of reflections | 32517 | |
| Completeness [%] | 99.5 | 96.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 22% PEG monomethyl ether 2,000, 0.15 M Potassium bromide, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
