3PJN
The crystal structure of Tp34 bound to Zn(II) ion at pH 7.5
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-12-08 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97926 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 34.573, 65.397, 152.359 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 32.900 - 1.700 |
R-factor | 0.1793 |
Rwork | 0.178 |
R-free | 0.20610 |
Structure solution method | FOURIER SYNTHESIS |
RMSD bond length | 0.010 |
RMSD bond angle | 1.342 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX ((phenix.refine: 1.6.4_486)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 32.900 | 1.760 |
High resolution limit [Å] | 1.700 | 1.700 |
Number of reflections | 38964 | |
<I/σ(I)> | 2.9 | |
Completeness [%] | 99.4 | 99.4 |
Redundancy | 4.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 9 | 293 | 0.1 M Bicine, 2.4 M Ammonium Sulfate, pH 9, VAPOR DIFFUSION, HANGING DROP, temperature 293K |