3PJ7
Crystal structure of far-red fluorescent protein Katushka crystallized at pH 8.5
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1.0 |
Spacegroup name | I 41 |
Unit cell lengths | 161.353, 161.353, 74.486 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 24.990 - 1.850 |
R-factor | 0.197 |
Rwork | 0.196 |
R-free | 0.25180 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.022 |
RMSD bond angle | 2.139 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | REFMAC |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 30.000 | 30.000 | 1.860 |
High resolution limit [Å] | 1.800 | 3.880 | 1.800 |
Rmerge | 0.096 | 0.066 | |
Number of reflections | 88163 | ||
<I/σ(I)> | 8.4 | ||
Completeness [%] | 100.0 | 100 | 100 |
Redundancy | 5 | 5 | 4.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 24% w/v PEG4000, 0.16 M MgCl2, 0.08 M TRIS hydrochloride pH 8.5, 20% v/v glycerol, VAPOR DIFFUSION, HANGING DROP, temperature 293K |