3PJ0
Crystal structure of a putative L-allo-threonine aldolase (lmo0305) from Listeria monocytogenes EGD-E at 1.80 A resolution
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-05-13 |
| Detector | MAR MAR325 |
| Wavelength(s) | 0.96109,0.97915,0.97936 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 85.593, 96.642, 99.478 |
| Unit cell angles | 90.00, 110.81, 90.00 |
Refinement procedure
| Resolution | 29.700 - 1.800 |
| R-factor | 0.163 |
| Rwork | 0.162 |
| R-free | 0.19800 |
| Structure solution method | MAD |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.488 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.5.0110) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.699 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.371 | |
| Number of reflections | 138522 | |
| <I/σ(I)> | 1.9 | |
| Completeness [%] | 94.4 | 95.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.36 | 277 | 53.80% 2-methyl-2,4-pentanediol, 0.1M TRIS pH 8.36, NANODROP, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
