3PIV
Zebrafish interferon 1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I911-3 |
| Synchrotron site | MAX II |
| Beamline | I911-3 |
| Temperature [K] | 77 |
| Detector technology | CCD |
| Collection date | 2009-06-12 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.8742 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 48.834, 62.247, 51.306 |
| Unit cell angles | 90.00, 91.42, 90.00 |
Refinement procedure
| Resolution | 48.819 - 2.086 |
| R-factor | 0.1855 |
| Rwork | 0.183 |
| R-free | 0.23500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.026 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.5_2)) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 51.290 | 48.832 | 2.217 |
| High resolution limit [Å] | 2.086 | 3.791 | 2.086 |
| Rmerge | 0.140 | ||
| Number of reflections | 18309 | ||
| <I/σ(I)> | 12.4 | ||
| Completeness [%] | 96.3 | 98 | 69 |
| Redundancy | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 295 | 0.2 M NaCl, 20% PEG3350, 0.1 M HEPES pH 6.5, 3% (v/v) Jeffamine M-600, VAPOR DIFFUSION, SITTING DROP, temperature 295K |
