3PIQ
Crystal structure of human 2909 Fab, a quaternary structure-specific antibody against HIV-1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-BM |
Synchrotron site | APS |
Beamline | 22-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-03-11 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 1 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 180.373, 180.373, 222.526 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 22.375 - 3.325 |
R-factor | 0.2397 |
Rwork | 0.236 |
R-free | 0.29910 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | PDB ID 3LRS |
RMSD bond length | 0.003 |
RMSD bond angle | 0.577 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.6.4_486)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.360 |
High resolution limit [Å] | 3.325 | 3.325 |
Number of reflections | 49978 | |
<I/σ(I)> | 8.8 | 1.95 |
Completeness [%] | 81.5 | 43.6 |
Redundancy | 5.1 | 2.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 17% PEG 8000, 0.1M Tris-Cl (pH 8.5), 0.1M NaCl, 3% galactose, VAPOR DIFFUSION, HANGING DROP, temperature 293K |