3PIL
Crystal structure of Mxr1 from Saccharomyces cerevisiae in reduced form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2008-12-26 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 64.310, 70.140, 74.030 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.170 - 2.040 |
| R-factor | 0.20858 |
| Rwork | 0.207 |
| R-free | 0.24665 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3bqe |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.828 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.170 | 2.140 |
| High resolution limit [Å] | 2.030 | 2.030 |
| Number of reflections | 21889 | |
| Completeness [%] | 98.4 | 89.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 4.6 | 289 | 0.2M ammonium acetate, 0.1M sodium acetate, 30% polyethylene glycol 4,000, pH 4.6, VAPOR DIFFUSION, temperature 289K |
