3PIH

T. maritima UvrA in complex with fluorescein-modified DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Detector technology	CCD
Collection date	2010-08-12
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.91841
Spacegroup name	P 42
Unit cell lengths	107.510, 107.510, 108.256
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.080 - 2.900
R-factor	0.2014
Rwork	0.198
R-free	0.26510
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2r6f
RMSD bond length	0.006
RMSD bond angle	1.046
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.6.4_486))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.950
High resolution limit [Å]	2.900	2.900
Rmerge	0.061	0.412
Number of reflections	19081
<I/σ(I)>		2.1
Completeness [%]	72.5	62.7
Redundancy	5.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	291	49-52% MPD, 0.1-0.2 M ammonium phosphate and 0.1 M Tris pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 291K