3PI9
Site-specific Glycosylation of Hemoglobin Utilizing Oxime Ligation Chemistry as a Viable Alternative to PEGylation
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 113 |
| Detector technology | IMAGE PLATE |
| Collection date | 2010-09-27 |
| Detector | RIGAKU |
| Wavelength(s) | 1.5 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 58.180, 74.010, 131.770 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 37.570 - 2.900 |
| R-factor | 0.26289 |
| Rwork | 0.261 |
| R-free | 0.29260 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1g0a |
| RMSD bond length | 0.032 |
| RMSD bond angle | 2.095 |
| Data reduction software | CrystalClear |
| Data scaling software | CrystalClear |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 65.890 | 3.000 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Number of reflections | 12599 | |
| Completeness [%] | 95.4 | 90.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Microbatch under Oil | 9.5 | PEG 2250 MME, pH 9.5, Microbatch under Oil |
