3PHT
Crystal structure of H74A mutant of Helicobacter Pylori NikR
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2010-08-27 |
Detector | RIGAKU RAXIS IV++ |
Wavelength(s) | 1.5418 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 73.174, 73.174, 114.441 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 61.650 - 2.040 |
R-factor | 0.19267 |
Rwork | 0.191 |
R-free | 0.22999 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3lgh residues 60-140 |
RMSD bond length | 0.024 |
RMSD bond angle | 2.093 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | REFMAC (5.6.0077) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 61.650 | 2.110 |
High resolution limit [Å] | 2.040 | 2.040 |
Rmerge | 0.122 | |
Number of reflections | 20487 | |
<I/σ(I)> | 25.8 | 1.1 |
Completeness [%] | 100.0 | 100 |
Redundancy | 24.3 | 22.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 295 | 12% PEG20K, 0.1M MES pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 295K |