3PHT
Crystal structure of H74A mutant of Helicobacter Pylori NikR
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2010-08-27 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 73.174, 73.174, 114.441 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 61.650 - 2.040 |
| R-factor | 0.19267 |
| Rwork | 0.191 |
| R-free | 0.22999 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3lgh residues 60-140 |
| RMSD bond length | 0.024 |
| RMSD bond angle | 2.093 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0077) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 61.650 | 2.110 |
| High resolution limit [Å] | 2.040 | 2.040 |
| Rmerge | 0.122 | |
| Number of reflections | 20487 | |
| <I/σ(I)> | 25.8 | 1.1 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 24.3 | 22.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 295 | 12% PEG20K, 0.1M MES pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 295K |
