3PH6
Bovine beta lactoglobulin crytsallized through ligandation of yttrium
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2009-06-24 |
Detector | MAR scanner 345 mm plate |
Wavelength(s) | 1.5418 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 41.060, 63.710, 134.160 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.640 - 2.530 |
R-factor | 0.21722 |
Rwork | 0.216 |
R-free | 0.26520 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.006 |
RMSD bond angle | 1.023 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Refinement software | REFMAC (5.5.0110) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.580 |
High resolution limit [Å] | 2.520 | 2.520 |
Rmerge | 0.097 | 0.650 |
Number of reflections | 21458 | 1522 |
<I/σ(I)> | 17 | 3 |
Completeness [%] | 95.0 | 92.4 |
Redundancy | 7.7 | 6.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Batch crystallization | 7 | 277 | 0.3 mM Yttrium, pH 7, Batch crystallization, temperature 277K |