3PH4
Clostridium thermocellum Ribose-5-Phosphate Isomerase B with d-allose
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 6C1 |
Synchrotron site | PAL/PLS |
Beamline | 6C1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-10-17 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 1.24 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 69.134, 69.134, 154.284 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 39.010 - 2.070 |
R-factor | 0.18599 |
Rwork | 0.184 |
R-free | 0.22004 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3he8 |
RMSD bond length | 0.026 |
RMSD bond angle | 2.018 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.200 |
High resolution limit [Å] | 2.070 | 2.100 |
Number of reflections | 25673 | |
Completeness [%] | 95.7 | 99.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 285 | 0.05M Tris pH 7.0, 10% PEG 8000, 0.15M Magnesium chloride, 0.2M Potassium chloride , VAPOR DIFFUSION, SITTING DROP, temperature 285K |