3PH3
Clostridium thermocellum Ribose-5-Phosphate Isomerase B with d-ribose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 6C1 |
| Synchrotron site | PAL/PLS |
| Beamline | 6C1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-10-17 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.24 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 69.033, 69.033, 154.583 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 39.030 - 2.070 |
| R-factor | 0.20128 |
| Rwork | 0.199 |
| R-free | 0.23758 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3he8 |
| RMSD bond length | 0.025 |
| RMSD bond angle | 2.006 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0110) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.200 |
| High resolution limit [Å] | 2.070 | 2.100 |
| Number of reflections | 26917 | |
| Completeness [%] | 100.0 | 99.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 285 | 0.05M Tris pH 7.0, 10% PEG 8000, 0.15M Magnesium chloride, 0.2M Potassium chloride, VAPOR DIFFUSION, SITTING DROP, temperature 285K |
