3PH2
Structure of the imidazole-adduct of the Phormidium laminosum cytochrome c6 Q51V variant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-03-05 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9794 |
| Spacegroup name | P 65 |
| Unit cell lengths | 45.616, 45.616, 64.707 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 25.030 - 1.400 |
| R-factor | 0.18764 |
| Rwork | 0.186 |
| R-free | 0.22227 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2v08 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.370 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.031 | 1.450 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Rmerge | 0.068 | 0.400 |
| Number of reflections | 15086 | |
| <I/σ(I)> | 18 | 3.3 |
| Completeness [%] | 97.7 | 97 |
| Redundancy | 6.1 | 4.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.6 | 291 | 0.4 M NaH2PO4/1.6 M K2HPO4, 0.1 M imidazole ph 8.0, 0.2 M NaCl., VAPOR DIFFUSION, HANGING DROP, temperature 291K |
