3PH2
Structure of the imidazole-adduct of the Phormidium laminosum cytochrome c6 Q51V variant
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-03-05 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9794 |
Spacegroup name | P 65 |
Unit cell lengths | 45.616, 45.616, 64.707 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 25.030 - 1.400 |
R-factor | 0.18764 |
Rwork | 0.186 |
R-free | 0.22227 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2v08 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.370 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.031 | 1.450 |
High resolution limit [Å] | 1.400 | 1.400 |
Rmerge | 0.068 | 0.400 |
Number of reflections | 15086 | |
<I/σ(I)> | 18 | 3.3 |
Completeness [%] | 97.7 | 97 |
Redundancy | 6.1 | 4.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.6 | 291 | 0.4 M NaH2PO4/1.6 M K2HPO4, 0.1 M imidazole ph 8.0, 0.2 M NaCl., VAPOR DIFFUSION, HANGING DROP, temperature 291K |