3PH1
The Crystal Structure of a Pathogenic Protein from the Xanthomonas campestris Reveals a New Tetrameric PilZ Domain Self-Assembled via a Unusual Helical Bundle
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | NSRRC BEAMLINE BL13B1 |
Synchrotron site | NSRRC |
Beamline | BL13B1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-06-30 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97973 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 58.840, 89.360, 87.340 |
Unit cell angles | 90.00, 105.40, 90.00 |
Refinement procedure
Resolution | 25.020 - 2.100 |
R-factor | 0.229 |
Rwork | 0.229 |
R-free | 0.25900 |
Structure solution method | MAD |
RMSD bond length | 0.007 |
RMSD bond angle | 1.400 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SOLVE |
Refinement software | CNS (1.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.190 |
High resolution limit [Å] | 2.100 | 2.110 |
Number of reflections | 101046 | |
Completeness [%] | 90.0 | 98.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 6 | 298 | 20% PEG 3350, pH 6, VAPOR DIFFUSION, temperature 298K |