3PGE
Structure of sumoylated PCNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 4.2.2 |
| Synchrotron site | ALS |
| Beamline | 4.2.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-10-06 |
| Detector | NOIR-1 |
| Wavelength(s) | 0.97 |
| Spacegroup name | F 4 3 2 |
| Unit cell lengths | 268.816, 268.816, 268.816 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.400 - 2.800 |
| R-factor | 0.2324 |
| Rwork | 0.231 |
| R-free | 0.25340 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1plq |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.450 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.6.4_486)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.438 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.089 | 0.433 |
| Number of reflections | 20836 | |
| <I/σ(I)> | 14.8 | 4.4 |
| Completeness [%] | 100.0 | 99.5 |
| Redundancy | 12.6 | 8.83 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 291 | 2.0M Ammonium Sulfate, 0.1M sodium Citrate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
