3PGB
Crystal structure of Aspergillus nidulans amine oxidase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2010-04-01 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.5418 |
| Spacegroup name | I 4 2 2 |
| Unit cell lengths | 179.861, 179.861, 148.178 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 38.440 - 2.450 |
| R-factor | 0.21291 |
| Rwork | 0.211 |
| R-free | 0.25409 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1w7c |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.047 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA (3.3.9) |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 38.440 | 40.218 | 2.560 |
| High resolution limit [Å] | 2.446 | 7.680 | 2.430 |
| Rmerge | 0.101 | 0.815 | |
| Number of reflections | 44740 | ||
| <I/σ(I)> | 7.1 | ||
| Completeness [%] | 97.4 | 99.2 | 82.5 |
| Redundancy | 7.7 | 7 | 7.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.4 | 298 | 0.1M sodium acetate (pH 4.4), 25% w/v PEG 2000 MME, 0.2M ammonium sulfate, vapor diffusion, hanging drop, temperature 298K |
