3PG8
Truncated form of 3-deoxy-D-arabino-heptulosonate 7-phosphate synthase from Thermotoga maritima
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
Synchrotron site | Australian Synchrotron |
Beamline | MX2 |
Temperature [K] | 110 |
Detector technology | CCD |
Collection date | 2010-03-03 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9537 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 53.510, 55.456, 166.935 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 38.570 - 2.000 |
R-factor | 0.158 |
Rwork | 0.155 |
R-free | 0.20940 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1rzm |
RMSD bond length | 0.006 |
RMSD bond angle | 0.995 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Refinement software | PHENIX (1.6.1_357) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 45.047 | 45.047 | 2.110 |
High resolution limit [Å] | 2.000 | 6.320 | 2.000 |
Rmerge | 0.038 | 0.293 | |
Rmeas | 0.044 | 0.337 | |
Rpim | 0.021 | 0.162 | |
Total number of observations | 3914 | 17558 | |
Number of reflections | 32385 | ||
<I/σ(I)> | 12.3 | 28.8 | 4.1 |
Completeness [%] | 94.5 | 88.2 | 96.3 |
Redundancy | 3.7 | 3.7 | 3.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 293.15 | 8%(w/v) PEG 4000, 0.1M sodium acetate, 0.02%(w/v) sodium azide, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 293.15K |