3PG6
The carboxyl terminal domain of human deltex 3-like
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-10-22 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.97949, 0.97946 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 75.323, 76.146, 138.753 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.700 |
| R-factor | 0.16558 |
| Rwork | 0.165 |
| R-free | 0.18621 |
| Structure solution method | SAD |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.546 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHELX (C/D/E) |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.730 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Number of reflections | 88378 | |
| <I/σ(I)> | 31.6279 | 7.4 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 7.4 | 2.73 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6 | 293 | 22% PEG3350, 0.2 M TRI-LITHIUM CITRATE PH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
