3PG1
MAP kinase LmaMPK10 from Leishmania major (1.95 angs resolution)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 108 |
Detector technology | IMAGE PLATE |
Collection date | 2010-08-04 |
Detector | MAR scanner 345 mm plate |
Wavelength(s) | 1.5418 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 81.260, 81.260, 129.380 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 28.730 - 1.950 |
R-factor | 0.1936 |
Rwork | 0.192 |
R-free | 0.22400 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.011 |
RMSD bond angle | 1.070 |
Data reduction software | MOSFLM |
Data scaling software | SCALA (3.3.15) |
Phasing software | AMoRE |
Refinement software | BUSTER-TNT |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 28.730 | 28.730 | 2.060 |
High resolution limit [Å] | 1.950 | 6.170 | 1.950 |
Rmerge | 0.018 | 0.537 | |
Total number of observations | 2863 | 15881 | |
Number of reflections | 31659 | ||
<I/σ(I)> | 15.8 | 30.4 | 1.4 |
Completeness [%] | 98.3 | 85.9 | 97.4 |
Redundancy | 3.5 | 2.9 | 3.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 12% PEG 4000, 0.1M Hepes, 4% isopropanol, 5% glycerol, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |