3PFS
PWWP Domain of Human Bromodomain and PHD finger-containing protein 3
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-08-18 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97924 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 68.412, 134.522, 30.226 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 33.631 - 1.900 |
R-factor | 0.2134 |
Rwork | 0.211 |
R-free | 0.25890 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2x4w |
RMSD bond length | 0.011 |
RMSD bond angle | 1.199 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | REFMAC (refmac_5.6.0081) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 33.631 | 33.631 | 1.970 |
High resolution limit [Å] | 1.900 | 4.090 | 1.900 |
Rmerge | 0.091 | 0.048 | 0.476 |
Number of reflections | 22879 | ||
<I/σ(I)> | 9.5 | ||
Completeness [%] | 99.9 | 99.3 | 100 |
Redundancy | 6 | 5.6 | 6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 30% PEG4000, 0.2M Ammonium Sulfate, 0.1M Sodium Cacodylate pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |