3PFR

Crystal structure of D-Glucarate dehydratase related protein from Actinobacillus Succinogenes complexed with D-Glucarate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X4A
Synchrotron site	NSLS
Beamline	X4A
Temperature [K]	100
Detector technology	CCD
Collection date	2010-10-22
Detector	ADSC QUANTUM 4
Wavelength(s)	0.97915
Spacegroup name	P 41
Unit cell lengths	85.712, 85.712, 253.238
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.864 - 1.899
R-factor	0.218
Rwork	0.216
R-free	0.26320
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.108
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	BALBES
Refinement software	PHENIX ((phenix.refine: 1.5_2))

Data quality characteristics

	Overall
Low resolution limit [Å]	38.864
High resolution limit [Å]	1.899
Number of reflections	140294
Completeness [%]	98.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	293	30% PEG 4000, 0.1M Tris-HCl, 0.2M lithium sulfate, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K