3PFQ
Crystal Structure and Allosteric Activation of Protein Kinase C beta II
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-B |
Synchrotron site | APS |
Beamline | 23-ID-B |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-12-04 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1.03318 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 114.270, 114.270, 170.840 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 57.100 - 4.000 |
R-factor | 0.2445 |
Rwork | 0.193 |
R-free | 0.24450 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | PDB entries 2I0E and 1A25 |
RMSD bond length | 0.012 |
RMSD bond angle | 0.878 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | CNS (1.3) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 57.100 | 4.220 |
High resolution limit [Å] | 4.000 | 4.000 |
Number of reflections | 10670 | |
Completeness [%] | 93.9 | 88 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 8.5 | 294 | 0.1M Tris, pH 8.5, 3% PEG8K, VAPOR DIFFUSION, temperature 294K |