3PFI
2.7 Angstrom resolution crystal structure of a probable holliday junction DNA helicase (ruvB) from Campylobacter jejuni subsp. jejuni NCTC 11168 in complex with adenosine-5'-diphosphate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-G |
| Synchrotron site | APS |
| Beamline | 21-ID-G |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-06-21 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.97856 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 111.482, 111.482, 355.089 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.590 - 2.695 |
| R-factor | 0.22427 |
| Rwork | 0.222 |
| R-free | 0.26796 |
| Structure solution method | SAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.033 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.750 |
| High resolution limit [Å] | 2.695 | 2.695 |
| Rmerge | 0.086 | 0.489 |
| Number of reflections | 23883 | |
| <I/σ(I)> | 16.32 | 3.44 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 4.6 | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 295 | Crystallization condition: Tacsimate 5%, PEG5KMME 10%, B-Tr 0.1M pH 5.5, AMPPNP 10mM. Cryo condition: 30% PEG 5KMME, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
