3PF6
The structure of uncharacterized protein PP-LUZ7_gp033 from Pseudomonas phage LUZ7.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-07-14 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 0.97923 |
| Spacegroup name | P 1 |
| Unit cell lengths | 31.886, 40.822, 46.268 |
| Unit cell angles | 70.14, 84.61, 83.86 |
Refinement procedure
| Resolution | 31.640 - 1.600 |
| R-factor | 0.1361 |
| Rwork | 0.135 |
| R-free | 0.16470 |
| Structure solution method | SAD |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.333 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MLPHARE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.630 |
| High resolution limit [Å] | 1.600 | 4.340 | 1.600 |
| Rmerge | 0.123 | 0.124 | 0.245 |
| Number of reflections | 27869 | ||
| <I/σ(I)> | 15.4 | ||
| Completeness [%] | 96.7 | 95.5 | 95.2 |
| Redundancy | 5.8 | 5.4 | 5.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.7 | 297 | 0.2M NaCl, 0.1M tri-sodium citrate pH 5.7, 29% PEG 3350, VAPOR DIFFUSION, HANGING DROP, temperature 297K |
