3PF2
The Crystal Structure of the Major Pilin GBS80 of Streptococcus agalactiae 35kDa C-terminal fragment
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2009-01-01 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 130.508, 34.658, 74.610 |
| Unit cell angles | 90.00, 93.29, 90.00 |
Refinement procedure
| Resolution | 40.000 - 1.700 |
| R-factor | 0.20463 |
| Rwork | 0.203 |
| R-free | 0.23798 |
| Structure solution method | SAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.223 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.760 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.028 | 0.170 |
| Number of reflections | 37173 | |
| <I/σ(I)> | 26.7 | 6.5 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 4.3 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 295 | 25% PEG MME 2000, 0.1M MES, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
