3PEU

S. cerevisiae Dbp5 L327V C-terminal domain bound to Gle1 H337R and IP6

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	100
Detector technology	CCD
Collection date	2009-11-13
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9796, 0.9798, 1.020
Spacegroup name	P 61 2 2
Unit cell lengths	110.356, 110.356, 201.453
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	48.393 - 2.600
R-factor	0.1884
Rwork	0.187
R-free	0.21320
Structure solution method	MAD
RMSD bond length	0.003
RMSD bond angle	0.677
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE (2.13)
Refinement software	PHENIX (1.6.4_486)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.690
High resolution limit [Å]	2.600	5.600	2.600
Rmerge	0.100	0.035	0.690
Number of reflections	42284
<I/σ(I)>	18.9
Completeness [%]	99.9	99.3	100
Redundancy	7.5	7.5	7.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	291	30% PEG 3350, 100 mM HEPES pH 8.0, 50 mM NaOAc, 200mM LiS04, 10 mM HEPES pH 7.5, 100mM NaCl, 1mM DTT, 0.5 mM IP6, 5% glycerol, VAPOR DIFFUSION, HANGING DROP, temperature 291K