3PES
Crystal structure of uncharacterized protein from Pseudomonas phage YuA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-06-11 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9794 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 38.059, 59.745, 38.154 |
| Unit cell angles | 90.00, 103.98, 90.00 |
Refinement procedure
| Resolution | 23.230 - 1.300 |
| R-factor | 0.17708 |
| Rwork | 0.176 |
| R-free | 0.20477 |
| Structure solution method | SAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.260 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.350 |
| High resolution limit [Å] | 1.300 | 1.300 |
| Rmerge | 0.090 | 0.450 |
| Number of reflections | 39941 | |
| <I/σ(I)> | 29 | 2.8 |
| Completeness [%] | 97.5 | 95.3 |
| Redundancy | 3.7 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 291 | 0.1 M SPG, 25 % Peg3350, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
