3PEI
Crystal Structure of Cytosol Aminopeptidase from Francisella tularensis
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-08-22 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97924 |
Spacegroup name | P 63 2 2 |
Unit cell lengths | 161.483, 161.483, 105.270 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 50.000 - 2.700 |
R-factor | 0.167 |
Rwork | 0.165 |
R-free | 0.21000 |
Structure solution method | SAD |
RMSD bond length | 0.011 |
RMSD bond angle | 1.339 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | HKL-3000 |
Refinement software | PHENIX |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.750 |
High resolution limit [Å] | 2.700 | 2.700 |
Number of reflections | 22700 | |
<I/σ(I)> | 10.2 | 5.8 |
Completeness [%] | 99.9 | 100 |
Redundancy | 10.4 | 10.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 289 | 0.2M potassium iodide, 20 % PEG 3350, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 289K |