3PEB
The Structure of a Creatine_N Superfamily domain of a dipeptidase from Streptococcus thermophilus.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-06-14 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 0.97937 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 70.503, 70.503, 160.388 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 34.430 - 1.860 |
| R-factor | 0.1713 |
| Rwork | 0.170 |
| R-free | 0.19040 |
| Structure solution method | SAD |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.395 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MLPHARE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.880 |
| High resolution limit [Å] | 1.850 | 5.020 | 1.850 |
| Rmerge | 0.074 | 0.066 | 0.782 |
| Number of reflections | 20449 | ||
| <I/σ(I)> | 9.9 | ||
| Completeness [%] | 99.4 | 98.8 | 94.6 |
| Redundancy | 23.8 | 22.1 | 15.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 9.5 | 297 | 0.1M CHES pH 9.5, 1.0M Sodium Citrate, VAPOR DIFFUSION, SITTING DROP, temperature 297K |
