3PDX
Crystal structural of mouse tyrosine aminotransferase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Wavelength(s) | 1.0809 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 69.275, 84.839, 157.992 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.940 - 2.910 |
| R-factor | 0.26516 |
| Rwork | 0.263 |
| R-free | 0.29522 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3dyd |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.718 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.000 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.086 | 0.388 |
| Number of reflections | 10641 | |
| <I/σ(I)> | 24.4 | 2.3 |
| Redundancy | 6 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.8 | 293 | 20% PEG 4000, 100 mM cacodylic acid buffer, and 20% glycerol. , pH 6.8, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
