3PBG

6-PHOSPHO-BETA-GALACTOSIDASE FORM-C

Experimental procedure

Source type	ROTATING ANODE
Source details	RIGAKU RUH2R
Temperature [K]	277
Detector technology	AREA DETECTOR
Collection date	1994-04
Detector	SIEMENS
Spacegroup name	P 21 21 2
Unit cell lengths	107.100, 174.500, 61.000
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 2.700
R-factor	0.174
Rwork	0.174
R-free	0.24200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1pbg
RMSD bond length	0.010
RMSD bond angle	24.400 *
Data reduction software	XDS
Data scaling software	MERGE
Phasing software	AMoRE
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall
Low resolution limit [Å]	10.000 *
High resolution limit [Å]	2.700 *
Rmerge	0.043 *
Number of reflections	24703 *
Completeness [%]	79.0 *
Redundancy	2.2 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.5		pH 7.5

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	12 (mg/ml)
2	1	drop		0.1 (M)
3	1	drop		0.02 (%)
4	1	reservoir	ammonium sulfate	1.9 (M)
5	1	reservoir	PEG600	2 (%)
6	1	reservoir		0.02 (%)
7	1	reservoir	Tris-HCl	0.1 (M)