3PBF
Surfactant Protein-A neck and carbohydrate recognition domain (NCRD) complexed with glycerol
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X8C |
| Synchrotron site | NSLS |
| Beamline | X8C |
| Temperature [K] | 90 |
| Detector technology | CCD |
| Collection date | 2002-08-15 |
| Detector | ADSC QUANTUM 4r |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 63 |
| Unit cell lengths | 97.376, 97.376, 44.905 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 23.390 - 1.800 |
| R-factor | 0.207 |
| Rwork | 0.207 |
| R-free | 0.22300 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | PDB CODE 1R13 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.100 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.044 | 0.229 |
| Number of reflections | 22680 | |
| <I/σ(I)> | 14.8 | 5.6 |
| Completeness [%] | 99.6 | 98.2 |
| Redundancy | 5 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 290 | 1ul SP-A (10 mg/ml) was mixed with 1ul reservoir (50 mM sodium cacodylate (pH = 6.5), 1.2-1.6 M lithium sulfate, 10 mM calcium chloride, and 5 % v/v glycerol. Prior to data collection, crystals were cryoprotected in a solution with higher amounts of glycerol., VAPOR DIFFUSION, HANGING DROP, temperature 290K |
