3PB2
Characterisation of the first monomeric dihydrodipicolinate synthase variant reveals evolutionary insights
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2006-01-01 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.95661 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 192.200, 125.342, 78.428 |
| Unit cell angles | 90.00, 103.54, 90.00 |
Refinement procedure
| Resolution | 33.190 - 1.900 |
| R-factor | 0.1707 |
| Rwork | 0.169 |
| R-free | 0.21040 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.012 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | PHENIX ((phenix.refine: 1.6.1_357)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 33.259 |
| High resolution limit [Å] | 1.900 |
| Number of reflections | 143667 |
| Completeness [%] | 96.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.2 | 293 | 40% v/v PEG 300, 100mM phosphate-citrate buffer, pH 4.2, 0.02% (w/v) sodium azide, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
