3PB2
Characterisation of the first monomeric dihydrodipicolinate synthase variant reveals evolutionary insights
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
Synchrotron site | Australian Synchrotron |
Beamline | MX2 |
Temperature [K] | 110 |
Detector technology | CCD |
Collection date | 2006-01-01 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.95661 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 192.200, 125.342, 78.428 |
Unit cell angles | 90.00, 103.54, 90.00 |
Refinement procedure
Resolution | 33.190 - 1.900 |
R-factor | 0.1707 |
Rwork | 0.169 |
R-free | 0.21040 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.007 |
RMSD bond angle | 1.012 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | AMoRE |
Refinement software | PHENIX ((phenix.refine: 1.6.1_357)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 33.259 |
High resolution limit [Å] | 1.900 |
Number of reflections | 143667 |
Completeness [%] | 96.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4.2 | 293 | 40% v/v PEG 300, 100mM phosphate-citrate buffer, pH 4.2, 0.02% (w/v) sodium azide, VAPOR DIFFUSION, SITTING DROP, temperature 293K |