3PAK
Crystal Structure of Rat Surfactant Protein A neck and carbohydrate recognition domain (NCRD) complexed with Mannose
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU300 |
Temperature [K] | 90 |
Detector technology | IMAGE PLATE |
Collection date | 2006-07-07 |
Detector | RIGAKU RAXIS IV |
Wavelength(s) | 1.5418 |
Spacegroup name | P 63 |
Unit cell lengths | 97.796, 97.796, 45.088 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 26.100 - 1.900 |
R-factor | 0.218 |
Rwork | 0.218 |
R-free | 0.24300 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 1r13 |
RMSD bond length | 0.004 |
RMSD bond angle | 1.100 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.940 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.050 | 0.287 |
Number of reflections | 19500 | |
<I/σ(I)> | 24.5 | 4.5 |
Completeness [%] | 99.4 | 98.9 |
Redundancy | 5 | 3.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 290 | 1 ul SP-A (10 mg/ml) was mixed with 1 ul reservoir (50 mM sodium cacodylate (ph = 6.5), 1.2-1.6 M lithium sulfate, and 10 mM calcium chloride). After crystals grew, the drop was diluted 1/2 with 50 mM sodium cacodylate (pH =6.5) and 10 mM calcium chloride. Then, mannose powder was added to the drop and mixed to dissolve. A second dilution by 1/2 again and addition of more mannose powder preceded data collection., VAPOR DIFFUSION, HANGING DROP, temperature 290K |