3PAB
Crystal Structure of H2-Kb in complex with a mutant of the chicken ovalbumin epitope OVA-E1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX1 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-03-27 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 0.954 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 66.683, 88.818, 89.121 |
| Unit cell angles | 90.00, 111.38, 90.00 |
Refinement procedure
| Resolution | 42.365 - 2.200 |
| R-factor | 0.1963 |
| Rwork | 0.193 |
| R-free | 0.26000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1vac |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.116 |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 100.000 | 2.300 | |
| High resolution limit [Å] | 2.200 | 10.000 | 2.200 |
| Rmerge | 0.094 | 0.041 | 0.380 |
| Rmeas | 0.043 | 0.400 | |
| Number of reflections | 49257 | 549 | 6155 |
| <I/σ(I)> | 18.49 | 47 | 5.9 |
| Completeness [%] | 99.9 | 98.9 | 100 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 277 | 0.2M calcium acetate, 15% PEG 8K, 0.1M Na-malonate, pH 6, vapor diffusion, hanging drop, temperature 277K |
