3PA6
Structure of the N-terminal BRCT domain of human microcephalin (MCPH1)
Experimental procedure
| Experimental method | SAD |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-10-10 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 35.069, 39.274, 106.639 |
| Unit cell angles | 90.00, 91.52, 90.00 |
Refinement procedure
| Resolution | 35.530 - 1.500 |
| R-factor | 0.146 |
| Rwork | 0.144 |
| R-free | 0.19000 |
| Structure solution method | SAD |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.339 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | HKL2Map |
| Refinement software | PHENIX ((phenix.refine: 1.6.1_357)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.530 | 1.550 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.059 | 0.431 |
| Number of reflections | 41554 | |
| <I/σ(I)> | 46.1 | 1.9 |
| Completeness [%] | 88.9 | 43.1 |
| Redundancy | 6.4 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 295 | 0.2M sodium succinate, 15% PEG 3350, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
