3P94
Crystal structure of a GDSL-like Lipase (BDI_0976) from Parabacteroides distasonis ATCC 8503 at 1.93 A resolution
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL11-1 |
| Synchrotron site | SSRL |
| Beamline | BL11-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-06-10 |
| Detector | MARMOSAIC 325 mm CCD |
| Wavelength(s) | 0.91837,0.97913 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 72.800, 58.360, 98.610 |
| Unit cell angles | 90.00, 98.12, 90.00 |
Refinement procedure
| Resolution | 48.810 - 1.930 |
| R-factor | 0.184 |
| Rwork | 0.183 |
| R-free | 0.20700 |
| Structure solution method | MAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 0.960 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | SHELX |
| Refinement software | BUSTER-TNT (BUSTER 2.8.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.812 | 2.000 |
| High resolution limit [Å] | 1.930 | 1.930 |
| Rmerge | 0.103 | 0.552 |
| Number of reflections | 60928 | |
| <I/σ(I)> | 10.17 | 2.4 |
| Completeness [%] | 98.2 | 86.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.86 | 293 | 30.40% polyethylene glycol 4000, 0.20M sodium acetate, 0.1M TRIS pH 8.86, NANODROP, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
