3P8V
Crystal structure of the archaeal asparagine synthetase A complexed with L-Aspartic acid
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-2 |
Synchrotron site | ESRF |
Beamline | ID23-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-05-01 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.87300 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 58.330, 61.500, 155.990 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.293 - 1.800 |
R-factor | 0.1775 |
Rwork | 0.176 |
R-free | 0.21810 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.005 |
RMSD bond angle | 0.882 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.6.2_432)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 |
High resolution limit [Å] | 1.800 | 1.800 |
Number of reflections | 51846 | |
Completeness [%] | 98 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 100mM Tris-HCl pH7, 0.2M NaCl and 32% PEG3350 and soak in 1 to 5mM aspartic acid, VAPOR DIFFUSION, SITTING DROP, temperature 293K |