3P8U

Crystal structure of mEosFP in its green state

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-3
Synchrotron site	ESRF
Beamline	ID14-3
Temperature [K]	100
Detector technology	CCD
Collection date	2007-07-16
Detector	ADSC QUANTUM 4
Wavelength(s)	0.931
Spacegroup name	P 21 21 21
Unit cell lengths	86.450, 96.820, 140.430
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.410 - 2.250
R-factor	0.23434
Rwork	0.232
R-free	0.28352
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1zux
RMSD bond length	0.005
RMSD bond angle	1.420
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	REFMAC (5.5.0072)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	56.796	2.500
High resolution limit [Å]	2.250	2.250
Number of reflections	74471
Completeness [%]	92.8	99.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	298	2.2 M ammonium sulfate, 0.1 M Tris-HCl, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K