3P8T

Crystal structure of the archaeal asparagine synthetase A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE BM30A
Synchrotron site	ESRF
Beamline	BM30A
Temperature [K]	100
Detector technology	CCD
Collection date	2006-06-03
Detector	MAR CCD 345
Wavelength(s)	0.978
Spacegroup name	P 21 21 21
Unit cell lengths	57.930, 61.380, 156.110
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.249 - 1.780
R-factor	0.1518
Rwork	0.150
R-free	0.19210
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	1.050
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.6.2_432))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	50.000
High resolution limit [Å]	1.780	1.780
Number of reflections	54061
Completeness [%]	99.6	99.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	293	100mM Tris-HCl pH7, 0.2M NaCl and 32% PEG3350, VAPOR DIFFUSION, SITTING DROP, temperature 293K