3P7N

Crystal structure of light activated transcription factor El222 from Erythrobacter litoralis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-1
Synchrotron site	SSRL
Beamline	BL9-1
Temperature [K]	100
Detector technology	CCD
Collection date	2008-12-03
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9795
Spacegroup name	P 1 21 1
Unit cell lengths	37.044, 81.595, 65.478
Unit cell angles	90.00, 94.57, 90.00

Refinement procedure

Resolution	34.595 - 2.100
R-factor	0.2659
Rwork	0.263
R-free	0.32850
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2PR5 1YIO
RMSD bond length	0.003
RMSD bond angle	0.779
Data reduction software	d*TREK
Data scaling software	d*TREK
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: dev_417))

Data quality characteristics

	Overall
Low resolution limit [Å]	36.930
High resolution limit [Å]	2.100
Rmerge	0.102
Number of reflections	22731
<I/σ(I)>	10.1
Completeness [%]	99.8
Redundancy	7.68

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	290	20% PEG 8000, 0.1M MOPS pH 7.0, 0.1M ammonium chloride, VAPOR DIFFUSION, HANGING DROP, temperature 290K