3P7M
Structure of putative lactate dehydrogenase from Francisella tularensis subsp. tularensis SCHU S4
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-10-16 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9791 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 78.740, 99.147, 83.345 |
| Unit cell angles | 90.00, 103.54, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.200 |
| R-factor | 0.18468 |
| Rwork | 0.182 |
| R-free | 0.23307 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3gvh |
| RMSD bond length | 0.021 |
| RMSD bond angle | 1.600 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.240 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.126 | 0.497 |
| Number of reflections | 62128 | |
| <I/σ(I)> | 14.9 | 2.73 |
| Completeness [%] | 99.2 | 100 |
| Redundancy | 4.1 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 0.2M NH4 dihydrogen Phosphate, 20% PEG3350, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
