3P7J
Drosophila HP1a chromo shadow domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-BM |
Synchrotron site | APS |
Beamline | 19-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-10-17 |
Detector | ADSC QUANTUM 210r |
Wavelength(s) | 0.9792 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 44.755, 56.545, 67.295 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 31.120 - 2.300 |
R-factor | 0.20094 |
Rwork | 0.199 |
R-free | 0.24613 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2fmm |
RMSD bond length | 0.019 |
RMSD bond angle | 1.421 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.340 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.047 | 0.475 |
Number of reflections | 8023 | |
<I/σ(I)> | 37.2 | 4.3 |
Completeness [%] | 99.4 | 98.8 |
Redundancy | 7.1 | 7.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 277 | 12% PEG400, 0.1 M HEPES. Cryoprotected in 12% PEG400, 0.1 M HEPES, 33% glycerol, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |