3P7H
Structure of the human Langerin carbohydrate recognition domain in complex with maltose
Replaces: 3BC6Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.1 |
Synchrotron site | BESSY |
Beamline | 14.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-11-15 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.9796 |
Spacegroup name | P 42 |
Unit cell lengths | 79.970, 79.970, 90.950 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.000 - 2.300 |
R-factor | 0.18318 |
Rwork | 0.181 |
R-free | 0.22863 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3p7g |
RMSD bond length | 0.012 |
RMSD bond angle | 1.360 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.400 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.188 | |
Number of reflections | 25136 | |
<I/σ(I)> | 25.6 | 7.3 |
Completeness [%] | 98.5 | 98.3 |
Redundancy | 4.1 | 4.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.9 | 293 | Protein: 10mg/ml Langerin in 10 mM Tris/HCl pH 7.5, 5 mM Maltose; Reservoir: 0.1 M Na-cacodylate, 13 %(w/v) PEG4000, 0.1 M MgCl2, 5mM CaCl2, VAPOR DIFFUSION, SITTING DROP, temperature 293K |